Bio-chemical analysis of Datura stramonium extract
DOI:
https://doi.org/10.24193/subbbiol.2017.2.01Keywords:
atropine, bacterial susceptibility, Datura stramonium, High Performance Liquid Chromatography with reversed phase, scopolamine, thin-layer chromatography.Abstract
Datura stramonium is an annual plant which belongs to the Solanaceae family. Datura stramonium is also a toxic plant, with toxicity given by the presence of alkaloids hyoscyamine, atropine and scopolamine.This study performs phytochemical and antibacterial analysis of ethanolic extracts from Datura stramonium. Fresh plants have been used in order to obtain Datura stramonium tincture. The quality index was 1:5, the concentration of ethanol being 90%. Based on this tincture a qualitative and quantitative phytochemical analysis was performed through thin layer chromatography and high performance liquid chromatography. By thin-layer chromatography the qualitative alkaloids, such as atropine and scopolamine from Datura stramonium extract, have been identified. By high performance liquid chromatography with reversed phase the alkaloids quantity from Datura stramonium herbs was assessed and that is 1.7 mg/mL. The plant extracts were tested on Gram negative bacteria Escherichia coli and on Gram positive bacteria Staphylococcus aureus. Both tested strains showed resistance but for E. coli a higher inhibition was observed at all samples containing Datura extract.
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