Plant growth response and nitrate reductase activities of roots of Chromolaena odorata in a model spent lubricating oil-polluted soil

Authors

  • Geoffrey O. ANOLIEFO Environmental Biotechnology and Sustainability Research Group, Dept of Plant Biology & Biotechnology, University of Benin, Benin City, Nigeria.
  • Beckley IKHAJIAGBE Environmental Biotechnology and Sustainability Research Group, Dept of Plant Biology & Biotechnology, University of Benin, Benin City, Nigeria. *Corresponding author: beckley.ikhajiagbe@uniben.edu https://orcid.org/0000-0003-2834-7447
  • Ehimwenma S. OMOREGIE Malaria Research, Molecular Biology and Toxicology Unit, Dept. of Biochemistry, University of Benin, Benin City, Nigeria. https://orcid.org/0000-0003-1751-6022
  • Raphael A. AIMIEBENOMOR Malaria Research, Molecular Biology and Toxicology Unit, Dept. of Biochemistry, University of Benin, Benin City, Nigeria.
  • Pascal C. OKOYE Environmental Biotechnology and Sustainability Research Group, Dept of Plant Biology & Biotechnology, University of Benin, Benin City, Nigeria. https://orcid.org/0000-0003-4283-3863

DOI:

https://doi.org/10.24193/subbbiol.2017.2.09

Keywords:

bioaccumulation, Chromolaena odorata, enzymes, nitrate assimilation, nitrate reductase, phytoremediation.

Abstract

The ability of Chromolaena odorata propagated by stem cuttings and grown for 50 days in the soil containing five (5) different concentrations of spent lubricating oil (SLO) in soil (0, 1, 3, 6, 9 and 12 % SLO), was investigated. The experiments were watered daily at 70% moisture field capacity. Parameters such as number leaves per plant, shoot length, plant height as well as nitrate reductase activities were measured. Shoot length as well as leaf number were significantly (p < 0.05) reduced, compared to the control treatment. Results also showed that nitrate reductase activities increased slightly with time. However, beyond 40 days, nitrate reductase activity was not detected in 3% - 12% w/w oil-in-soil treatments, respectively. Pollution indices such as Contamination factor and Hazard Quotient, used in the present study indicated significant reduction in contamination values upon sowing of C. Odorata. Values obtained from Bioaccumulation Quotients also indicated that the plant was able to significantly bioaccumulate elements such as Fe, Cu and Ni present in the SLO-polluted soil.

Anoliefo et al (PDF)

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Published

2017-12-20

Issue

Vol. 62 No. 2 (2017)

Section

Research article

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