Procedures of partial purification for phycobiliproteins from cyanobacteria isolated from soils of Republic of Moldova
DOI:
https://doi.org/10.24193/subbbiol.2018.1.01Keywords:
antioxidant capacity, cyanobacteria, microcystins, phycobiliproteins.Abstract
Investigation of the new cyanobacterial strains, for use as potential sources of bioactive substances, including phycobiliproteins, encounters some difficulties due to presence of toxins (microcystins) produced by some cyanobacterial strains. Cyanobacteria phycobiliproteins are natural pigments with high potential for application as colorants in food, cosmetics and pharmaceuticals. The objective of the study was the elaboration of a procedure for Anabaena propinqua Setchell. et Gardn. phycobiliproteins separation from microcystins and a procedure of partial purification of phycobiliproteins from cyanobacteria Anabenopsis sp. The antioxidant capacity of partial purified phycoerythrin from Anabenopsis sp. was established.
References
Abed, R. M., Dobretsov, S., Sudesh, K. (2009) Applications of cyanobacteria in biotechnology, J. Appl. Microbiol.106 (1):1-12
Bulimaga, V., Djur, S., Pisov, M., Rudi, L. Rudic, V. (2012) Antioxidant capacity of phycocyanin preparations obtained from Ge-enriched Spirulina biomass, Studia Universitatis Moldaviae, Ştiinţe reale şi ale naturii, 1 (51): 9-13 [in Romanian]
Bulimaga, V., Şalaru V. M., Zosim, L., Pisov, M., Trofim, A. (2014) Biochemical composition of the blue-green algae Anabaena propinqua at cultivation on the Drew nutritive media with and without nitrogen sources, 2nd International Conference on microbial technology. Octomber 9-10, Chisinau Moldova, pp.106
Bulimaga, V., Şalaru, V., Zosim, L., Pisov, M., Trofim, A. (2014) Blue-green algae (Cyanophyta) - sources of bioactive secondary metabolites, Studia Universitatis Moldaviae, Ştiinţe reale şi ale naturii, 1 (71): 96-107 [in Romanian]
Chakdar, H., Pabbi, S. (2012) Extraction and purification of phycoerythrin from Anabaena variabilis (CCC421), Phykos, 42 (1): 25 – 31
Chu, W. -L. (2012) Biotechnological applications of microalgae, Ie JSME., 6(Suppl1): S24-S37
Drogui, P., Daghrir, R., Simard, M.C., Sauvageau, C., Blais, J. F. (2012) Removal of microcystin-LR from spiked water using either activated carbon or anthracite as filter material, Environ Technol., 33(4-6):381-91
Ehmann, A., Guthrie, J. (2011) Methods for removal of microcystins and isolation of phycocyanin from Cyanobacteria. Patent. WO2011011174, Publication date 27.01.2011
Ehmann, A., Guthrie, J. (2015) Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria, Patent US 9131724 B2, Publication date 15.09.2015
Khatoona , H., Leong, L. K. , Rahman N. A., Mian S., Begum, H., Banerjee S. Endut, A. (2018) Effects of different light source and media on growth and production of phycobiliprotein from freshwater cyanobacteria, Bioresource Technology, 249: 652–658
Lau, N. -S., Matsui, M., Abdullah, A. A. -A. (2015) Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products, BioMed Research International, Article ID 754934, http://dx.doi.org/10.1155/2015/754934
Madamwar, D., Patel, D. K., Desai, S. N., Upadhyay, K. K., Devkar, R. V. (2015) Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells, EXCLI J., 14: 527–539
Manirafasha, E., Ndikubwimana T., Zeng , X., Lu, Y., Jing, K. (2016) Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent, Biochemical Engineering Journal, 109: 282–296
Pandey, H. G., Bano, F., Fatma, T. (2011) Studies on Anabaena sp. NCCU-9 with special reference to phycocyanin, J. Algal Biomass Utilization, 2(1): 30–51
Pyo, D., Moon, D. (2005) Adsorption of microcystin LR by activated carbon fibers, Bulletin of the Korean Chemical Society, 26 (12):2089-2092
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic Biol Med., 26 (9-10):1231-7
Sonani, R. R., Patel, S., Bhastana, B., Jakharia, K., Chaubey, M. G., Singh, N. K., Madamwar, D. (2017) Purification and antioxidant activity of phycocyanin from Synechococcus sp. R42DM isolated from industrially polluted site, Bioresource Technology, 245 (Pt A):325-331
Viskari, P. J., Colyer C. L. (2003) Rapid extraction of phycobiliproteins from cultured cyanobacteria samples, Anal Biochem. 319(2):263-71
Yan, H., Gong, A., He, H., Zhou, J., Wei, Y., Lv, L. (2006) Adsorption of microcystins by carbon nanotubes, Chemosphere, 62 (1):142-148
Published
Issue
Section
License
Copyright (c) 2018 Studia Universitatis Babeș-Bolyai Biologia. Published by Babeş-Bolyai University.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.