Procedures of partial purification for phycobiliproteins from cyanobacteria isolated from soils of Republic of Moldova

Authors

  • Valentina BULIMAGA SRL “Phycobiotechnology”, Moldova State University, Chișinău, Republic of Moldova, 65A. M. Kogălniceanu Street, MD 2009.
  • Maria PISOVA SRL “Phycobiotechnology”, Moldova State University, Chișinău, Republic of Moldova, 65A. M. Kogălniceanu Street, MD 2009.
  • Liliana ZOSIM SRL “Phycobiotechnology”, Moldova State University, Chișinău, Republic of Moldova, 65A. M. Kogălniceanu Street, MD 2009.
  • Alina TROFIM SRL “Phycobiotechnology”, Moldova State University, Chișinău, Republic of Moldova, 65A. M. Kogălniceanu Street, MD 2009. *Corresponding author: alinatrofim@yahoo.com

DOI:

https://doi.org/10.24193/subbbiol.2018.1.01

Keywords:

antioxidant capacity, cyanobacteria, microcystins, phycobiliproteins.

Abstract

Investigation of the new cyanobacterial strains, for use as potential sources of bioactive substances, including phycobiliproteins, encounters some difficulties due to presence of toxins (microcystins) produced by some cyanobacterial strains. Cyanobacteria phycobiliproteins are natural pigments with high potential for application as colorants in food, cosmetics and pharmaceuticals. The objective of the study was the elaboration of a procedure for Anabaena propinqua Setchell. et Gardn. phycobiliproteins separation from microcystins and a procedure of partial purification of phycobiliproteins from cyanobacteria Anabenopsis sp. The antioxidant capacity of partial purified phycoerythrin from Anabenopsis sp. was established.

Bulimaga et al (PDF)

References

Abed, R. M., Dobretsov, S., Sudesh, K. (2009) Applications of cyanobacteria in biotechnology, J. Appl. Microbiol.106 (1):1-12

Bulimaga, V., Djur, S., Pisov, M., Rudi, L. Rudic, V. (2012) Antioxidant capacity of phycocyanin preparations obtained from Ge-enriched Spirulina biomass, Studia Universitatis Moldaviae, Ştiinţe reale şi ale naturii, 1 (51): 9-13 [in Romanian]

Bulimaga, V., Şalaru V. M., Zosim, L., Pisov, M., Trofim, A. (2014) Biochemical composition of the blue-green algae Anabaena propinqua at cultivation on the Drew nutritive media with and without nitrogen sources, 2nd International Conference on microbial technology. Octomber 9-10, Chisinau Moldova, pp.106

Bulimaga, V., Şalaru, V., Zosim, L., Pisov, M., Trofim, A. (2014) Blue-green algae (Cyanophyta) - sources of bioactive secondary metabolites, Studia Universitatis Moldaviae, Ştiinţe reale şi ale naturii, 1 (71): 96-107 [in Romanian]

Chakdar, H., Pabbi, S. (2012) Extraction and purification of phycoerythrin from Anabaena variabilis (CCC421), Phykos, 42 (1): 25 – 31

Chu, W. -L. (2012) Biotechnological applications of microalgae, Ie JSME., 6(Suppl1): S24-S37

Drogui, P., Daghrir, R., Simard, M.C., Sauvageau, C., Blais, J. F. (2012) Removal of microcystin-LR from spiked water using either activated carbon or anthracite as filter material, Environ Technol., 33(4-6):381-91

Ehmann, A., Guthrie, J. (2011) Methods for removal of microcystins and isolation of phycocyanin from Cyanobacteria. Patent. WO2011011174, Publication date 27.01.2011

Ehmann, A., Guthrie, J. (2015) Methods for removal of microcystins and isolation of phycocyanin from cyanobacteria, Patent US 9131724 B2, Publication date 15.09.2015

Khatoona , H., Leong, L. K. , Rahman N. A., Mian S., Begum, H., Banerjee S. Endut, A. (2018) Effects of different light source and media on growth and production of phycobiliprotein from freshwater cyanobacteria, Bioresource Technology, 249: 652–658

Lau, N. -S., Matsui, M., Abdullah, A. A. -A. (2015) Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products, BioMed Research International, Article ID 754934, http://dx.doi.org/10.1155/2015/754934

Madamwar, D., Patel, D. K., Desai, S. N., Upadhyay, K. K., Devkar, R. V. (2015) Apoptotic potential of C-phycoerythrin from Phormidium sp. A27DM and Halomicronema sp. A32DM on human lung carcinoma cells, EXCLI J., 14: 527–539

Manirafasha, E., Ndikubwimana T., Zeng , X., Lu, Y., Jing, K. (2016) Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent, Biochemical Engineering Journal, 109: 282–296

Pandey, H. G., Bano, F., Fatma, T. (2011) Studies on Anabaena sp. NCCU-9 with special reference to phycocyanin, J. Algal Biomass Utilization, 2(1): 30–51

Pyo, D., Moon, D. (2005) Adsorption of microcystin LR by activated carbon fibers, Bulletin of the Korean Chemical Society, 26 (12):2089-2092

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay, Free Radic Biol Med., 26 (9-10):1231-7

Sonani, R. R., Patel, S., Bhastana, B., Jakharia, K., Chaubey, M. G., Singh, N. K., Madamwar, D. (2017) Purification and antioxidant activity of phycocyanin from Synechococcus sp. R42DM isolated from industrially polluted site, Bioresource Technology, 245 (Pt A):325-331

Viskari, P. J., Colyer C. L. (2003) Rapid extraction of phycobiliproteins from cultured cyanobacteria samples, Anal Biochem. 319(2):263-71

Yan, H., Gong, A., He, H., Zhou, J., Wei, Y., Lv, L. (2006) Adsorption of microcystins by carbon nanotubes, Chemosphere, 62 (1):142-148

Published

2018-06-22

Issue

Section

Research article