Assessment of enhanced biodegradation potentials of animal wastes on diesel-contaminated soil
DOI:
https://doi.org/10.24193/subbbiol.2020.2.02Keywords:
Diesel oil pollution, animal wastes, total petroleum hydrocarbon, plasmid profile.Abstract
Oil spillage is a menace, crippling most oil-producing regions around the globe. The aim of this study was to access the role of poultry litter and cow dung in enhancing biodegradation of diesel-contaminated soil. The treatment sets were split into three levels of diesel pollution (50 mL, 100 mL and 150 mL) amended with poultry litters, cow dung and a mixture of both amendments. The microbiological properties-and the total petroleum hydrocarbon content was analyzed for a period of six months using the pour plate techniques and Gas Chromatography (GC-FID), respectively. Agarose gel electrophoresis was used for plasmid detection. Mean total heterotrophic bacterial counts ranged between 40.5±0.5 x104 cfu-1 and 102.0 ±4.0 x104 cfu-1, for C1 (soil with poultry litter and cow dung with 50mL diesel) and Control 2. The mean total hydrocarbonoclastic bacterial counts ranged from 42.0±2.0 x104 cfu-1 to 66.5±2.5 x104 cfu-1 for B1 (soil with cow dung with 50mL diesel) and C3 (soil with poultry litter and cow dung with 150mL diesel). Bacillus subtilis (25.7%) and Staphylococcus aureus (4.73%) were reported as the isolates with the highest and least percentage frequency of occurrence. The percentage of diesel oil degradation was highest in C1 (98.5%) and lowest in Control 1 (31.6%). Plasmid extraction studies carried revealed that two out of the five hydrocarbonoclastic bacteria had both plasmids and chromosomal genes. The result has indicated the enhanced capacity of mixed amendments relative to individual waste treatment used in this study and should be recommended for bioremediation application.
Article history: Received 11 February 2020; Revised 16 November 2020; Accepted 4 December 2020; Available online 20 December 2020.
References
Adebusoye, S.A., Ilori, M. O., Amund, O. O., Teniola, O.D., & Olatope, S.O. (2007). Microbial degradation of petroleum hydrocarbons in a polluted tropical stream. World Journal of Microbiology and Biotechnology 23:1149-1159.
Adelana, S.O, Adeosun, T.A, Adesina, A.O., & Ojuroye, M.O. (2011). Environmental pollution and remediation: challenges and management of oil spillage in the Nigerian coastal area. American Journal of Scientific and Industrial Research (AJSIR) 2(6): 834-845.
Akpes, A.R., Ekundayo, A.O., & Esumeh, F.I. (2013). Degra.dation of crude oil by bacteria: A role for plasmid-borne genes. Global Journal of Science Frontier Research Biological Science, 13(6):20-26..
Chikere, C.B., & Azubuike, C.C. (2014). Characterization of hydrocarbon utilizing fungi from hydrocarbon polluted sediments and water Nigerian Journal of Biotechnology, 27:49-54.
Crosa, J.H., Tolmasky, M.E., Actis, L.A., & Falkow, S. (1994). Plasmids. In: Gerhardt, P., Murray, R.G.E., Wood, W.A., Kreig. N.R., editors. Methods for General and Molecular Bacteriology. American Society for Microbiology, Washington, 365-386.
De Magalhaes, J.T, Uetanabaro, P.T., & Moraes, C.A. (2008). Identification of Lactobacillus UFV H2B2 (Proboitic strain) using DNA-DNA hybridization. Brazilian Journal of Microbiology, 39:524-546.
Holt, J.G., Kreig, N. R., Sneath, P.H.A., Stanley, J.T., & Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology. 9th edition. Lippincott. Williams and Wilkins, Baltimore, USA. 787pp.
John, R.C., & Okpokwasili, G.C. (2012). Crude Oil-Degradation And Plasmid Profile Of Nitrifying Bacteria Isolated From Oil-Impacted Mangrove Sediment in the Niger Delta of Nigeria. Bulletin of Environmental Contamination and Toxicology 88:1020–1026
Mohania, D., Nagpal, R., Kumar, M., Bhardwaj, A., Yadav, M., Jain, S., Marotta, F., Singh, V., Parkash, O. & Yadav, H. (2008). Molecular approachhes for identification and chacterization of Lactic acid bacteria. Journal of Digestive Discussion, 9:190-198.
Obasi, N.A., Eze, E., Anyanwu, D.I., & Okorie, U.C. (2013). Effects of organic manures on the physicochemical properties of crude oil polluted soils. African Journal of Biochemistry Research, 7(6):67-75.
Omotayo, A.E., Ojo, O.Y., & Amund O.O. (2012). Crude oil degradation by microorganisms in soil compost. Research Journal of Microbiology, (4):209-218.
Onyeonwu R.O. (2000). Manual for Waste/Wastewater; Soil Sediment, Plant and Fish Analysis. Macgill Environmental Research Laboratory Manual. Benin City. 81pp.
Pepper, I. L., & Gerba, C. P. (2004). Environmental Microbiology: A laboratory Manual. 2nd edition. Elsevier Academic Press, London. 226pp.
Sharma, P. (2009). Manual of Microbiology, Tools and Techniques. Ane books. Pvt. Ltd. New Delhi, 405pp.
Sheikh, A.R., Afsheen A, Sadia, K., & Abdu, W. (2003). Plasmid borne antibiotic resistance factors among indigenous Klebsiella. Pakistan Journal of Biological Science, 35(2): 243–248
Stephen, E., Okwute, L.O., & Okai, A.I. (2015). Bioremediation of mechanic workshop polluted soil amended with poultry litter. Bioscience Research in Today’s World, 1(1):77-83.
Tanee, F.B.G., & Kinako, P.D.S. (2008). Comparative Studies of Biostimulation and phytoremediation in the mitigation of crude oil toxicity in tropical soil. Journal of Applied Science and Environmental Management, 12(2):143-147.
Umar, A.F., Tahir, F., Larkin, M., Oyawoye, O.M., Musa, B. L., Yerima, M.B., & Agbo, E.B. (2012). In-situ biostimulatory effect of selected organic wastes on bacterial atrazine biodegradation. Advances in Microbiology, 2:587-592.
Published
Issue
Section
License
Copyright (c) 2020 Studia Universitatis Babeș-Bolyai Biologia. Published by Babeș-Bolyai University.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
This work is licensed under a 