Lipid classes and fatty acid composition of Thapsia garganica L. oil seeds
DOI:
https://doi.org/10.24193/subbbiol.2024.1.05Keywords:
Thapsia garganica, fatty acids, petroselinic acid, pentadecanoic acid, tocopherols, antioxidant activityAbstract
This study focused on characterizing the seed oil of Thapsia garganica (Apiaceae), a medicinal plant native to Laghouat, Algeria, and evaluating its antioxidant properties. Various solvent systems were employed to extract and fractionate the lipid content of T. garganica seeds oil. Chemical indices were determined, and fatty acids methyl esters were analyzed using GC/MS. Tocopherol composition was assessed via HPLC, and antioxidant activity was evaluated using the 2,2-diphényl 1-picrylhydrazyle (DPPH) method. The GC/MS analysis revealed distinct fatty acid profiles across various fractions, highlighting a notable presence of petroselinic acid and higher-than-usual levels of pentadecanoic acid in all fractions. T. garganica oil exhibited richness in tocopherols, particularly with α-tocopherol being the predominant homolog. The antioxidant activity assessment of different lipid fractions indicated potent activity within polar lipids (glycolipids and phospholipids). Furthermore, T. garganica oil was abundant in unsaturated fatty acids, notably petroselinic acid, displaying significant radical scavenging activity in its polar fractions.
References
AFNOR (1984). Recueil de normes françaises des corps gras, graines oléagineuses, produits dérivés. AFNOR Paris.
Aït Youssef, M. (2006). Plantes médicinales de Kabylie. Éditions Ibis press Paris.
Avato, P., Fanizzi, F.P., & Rosito, I. (2001). The genus Thapsia as a source of petroselinic acid. Lipids, 36(8), 845-850.
Boukouada, M., Ghiaba, Z., Gourine, N., Bombarda, I., Saidi, M., & Yousfi, M. (2014). Chemical composition and antioxidant activity of seed oil of two Algerian date palm cultivars (Phoenix dactylifera). Nat Prod Commun, 9(12), 1777-80.
Cahoon, E.B., Shanklin, J., & Ohlrogge, J.B. (1992). Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. Proc Natl Acad Sci, 89(23), 11184-11188.
Folch, J., Lees, M., & Sloane Stanley, G.H. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J biol Chem, 226(1), 497-509.
Heywood, V.H. (1971). The biology and chemistry of the Umbelliferae. Academic Press for the Linnean Society London.
Jäger, A.K., Schottländer, B., Smitt, U.W., & Nyman, U. (1993). Somatic embryogenesis in cell cultures of Thapsia garganica: correlation between the state of differentiation and the content of thapsigargins. Plant Cell Rep, 12, 517-520.
Karleskind, A. (1992). Manuel des corps gras. Technique et Documentation-Lavoisier Paris.
Makunga, N.P., Jäger, A.K., & Van Staden, J. (2003). Micropropagation of Thapsia garganica—a medicinal plant. Plant Cell Rep, 21, 967-973.
Matthäus, B., &Özcan, M.M. (2015). Oil content, fatty acid composition and distributions of vitamin-E-active compounds of some fruit seed oils. Antioxidants, 4(1), 124-133.
Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol, 26(2), 211-219.
Nebeg, H., Benarous, K., Serseg, T., Lazreg, A., Hassani, H., & Yousfi, M. (2019). Seeds, leaves and roots of Thapsia garganica as a source of new potent lipases inhibitors: in vitro and in silico studies. Endocr Metab Immune Disord Drug Targets, 19(5), 683-696.
Ngo‐Duy, C.C., Destaillats, F., Keskitalo, M., Arul, J., & Angers, P. (2009). Triacylglycerols of Apiaceae seed oils: Composition and regiodistribution of fatty acids. Eur J Lipid Sci Technol, 111(2), 164-169.
Pinzi, S., Gandía, L.M., Arzamendi, G., Ruiz, J.J., & Dorado, M.P. (2011). Influence of vegetable oils fatty acid composition on reaction temperature and glycerides conversion to biodiesel during transesterification. Biores Technol, 102(2), 1044-1050.
Rossell, J.B., & Pritchard, J.L.R. (1991). Analysis of oilseeds, fats, and fatty foods. Elsevier Applied Science New York.
Rouser, G., Kritchevsky, G., & Yamamoto, A. (1967). Column chromatographic and associated procedures for separation and determination of phosphatides and glycolipids, In: Lipid chromatographic analysis, Marinetti G. V., Ed., Vol. 1, Marcel Dekker, New York, pp. 99-162.
Wolff, R.L. (1995). Recent applications of capillary gas-liquid chromatography to some difficult separations of positional or geometrical isomers of unsaturated fatty acids, In: New Trends in Lipid and Lipoprotein Analyses, Sébédio, J.-L., & Perkins, E.G., eds., AOCS Press, Champaign, pp. 147–180.
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